Gel filtration. Biuret method for determination of total protein in cerebrospinal fluid.
نویسندگان
چکیده
Interfering low molecular weight substances were removed from cerebro spinal fluid by gel filtration using Sephadex G-50-80 and total protein concen tration of the effluent was determined by the biuret reaction. Results of the proposed method were higher than comparable results obtained by a trichloro acetic acid turbidimetric method. Reproducibility of the method is acceptable.
منابع مشابه
Assessment of a biuret method without concentration step for total protein determination in cerebrospinal fluid.
E xamination of cerebrospinal fluid (CSF) total protein is used chiefly to detect increased permeabili ty of the blood-brain barrier to plasma proteins or increased intrathecal secretion of immunoglobulins (1). A rapid and quite precise method is required. The biuret method is considered as a potential reference method for total protein determination in biological fluids (2-4). It has been modi...
متن کاملCombined gel filtration, biuret/copper method compared with an immunochemical method for urinary protein measurement.
We compared an immunochemical method specific for plasma proteins with a chemical method, in which interfering substances are separated by gel filtration and "total" urinary protein is determined by the biuret reaction followed by reaction of protein-bound copper with diethyldithiocarbamate after a second gel filtration to remove nonprotein-bound copper. More than 250 24-h urine samples were an...
متن کاملDetermination of total urinary protein by combined gel filtration and automated biuret reaction.
Determination of total urinary protein is now more frequently requested of the clinical chemistry laboratory, one reason being the increased number of patients with renal transplants for whom total urinary protein is determined as an index of immunological rejection (1). Available manual methods are cumbersome and inefficient under clinical laboratory conditions. Concentrations for total urinar...
متن کاملThe direct spectrophotometric determination of protein in cerebrospinal fluid.
A rapid and sensitive direct spectrophotometric method for the determination of cerebrospinal fluid protein which usesas little as 0.1 ml. of sample has been described. The result is not affected by the ratio of albumin to globulin in the sample. Interfering substances are removed prior to spectrophotometric measurement with an automatic separation chamber employing the principle of gel filtrat...
متن کاملAssay of cerebrospinal fluid protein: a rate biuret method evaluated.
We evaluated a rate colorimetric method (Beckman) for measuring total protein in cerebrospinal fluid. The automated instrument we used was Beckman's ASTRA TM. A 100-microL sample of spinal fluid is introduced into the biuret reagent in the reaction cell and the increase in absorbance at 545 nm is monitored for 20.5 s. Solid-state circuits determine the rate of alkaline biuret-protein chelate fo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Annals of clinical and laboratory science
دوره 4 1 شماره
صفحات -
تاریخ انتشار 1974